Anti-Histone H2A.Z antibody - N-terminal (ab175552) at 1/1000 dilution + MDA-MB231 at 15 µg
Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue labeling H2A.Z with ab175552 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells labeling H2A.Z with ab175552 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.