Anti-Histone H2B (acetyl ) antibody - ChIP Grade
| Name | Anti-Histone H2B (acetyl ) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab1759 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC IHC-P IHC-F ChIP ELISA IP WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Drosophila, Toxoplasma gondii |
| Antigen | Synthetic peptide corresponding to Histone H2B aa 9-20 |
| Description | Rabbit Polyclonal |
| Gene | HIST2H2BF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab1759 staining Histone H2B (acetyl) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1759, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab1759 staining Histone H2B in Mouse hippocampus tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.1% Triton-X and blocked with 3% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/250 in TBS + 0.1% Triton-X 100 + 3% Goat serum) at 4°C for 12 hours. An Alexa Fluor® 546-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
ab1759 at a dilution of 1/200 staining the Female lymphoblastoid cell line, GM12616, by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected with a polyclonal goat anti-rabbit FITC. The DAPI counterstain is shown pseudocoloured as white in (b). A pale staining chromosome could be identified based on the low level of histone H2B acetylation of the inactive X chromosome. An enlargement of the pale staining chromosome is shown with DAPI pseudocoloured as red for contrast against the antibody (green).See Abreview
![Image from Bougdour A et al, J Exp Med 206:953-66 (2009), Fig S2.ab1759 used in Western Blot.FR235222 treatment specifically affects the acetylation status of histone H4 but not the acetylation levels of H3 and H2B. Extracellular T. gondii parasites (RH WT, R20D9 [TgHDAC3T99A], and M3135E11 [TgHDAC3T99I]) were treated with the indicated concentrations of FR235222 for 4 hours and lysed. 50µg of total cell lysates were analyzed by Immunoblotting with an anti-AcH4, anti-AcH2B (ab1759 at a 1/1000 dilution), anti-H3 and anti–alpha-tubulin antibodies.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/1270_Histone-H2B-Primary-antibodies-ab1759-1.jpg)
Image from Bougdour A et al, J Exp Med 206:953-66 (2009), Fig S2.ab1759 used in Western Blot.FR235222 treatment specifically affects the acetylation status of histone H4 but not the acetylation levels of H3 and H2B. Extracellular T. gondii parasites (RH WT, R20D9 [TgHDAC3T99A], and M3135E11 [TgHDAC3T99I]) were treated with the indicated concentrations of FR235222 for 4 hours and lysed. 50µg of total cell lysates were analyzed by Immunoblotting with an anti-AcH4, anti-AcH2B (ab1759 at a 1/1000 dilution), anti-H3 and anti–alpha-tubulin antibodies.
ab1759 staining Histone H2B (acetyl) in HeLa cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/200) for 1 hour at 20°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Gating Strategy: isotype population (shown in white).See Abreview
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