HIST2H2BF

Gene Symbol HIST2H2BF
Entrez Gene 440689
Alt Symbol -
Species Human
Gene Type protein-coding
Description histone cluster 2, H2bf
Other Description histone H2B type 2-F
Swissprots A8K0U9 Q5QNW6 B4DLA9
Accessions CAI12558 Q5QNW6 AK092418 BAC03883 AK289664 BAF82353 AK296916 BAG59471 AK299108 BAG61164 BC110793 AAI10794 DA042901 XM_006711335 XP_006711398 XM_006711336 XP_006711399 XM_006711337 XP_006711400 NM_001024599 NP_001019770 NM_001161334 NP_001154806
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus. Chromosome.
Top Pathways Systemic lupus erythematosus, Viral carcinogenesis, Alcoholism

Anti-HIST2H2BF antibody - ChIP Grade - ab94431 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ChIP analysis using ab94431 (0.5µg/mL) binding HIST2H2BF in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.

Anti-Histone H2B (acetyl ) antibody - ChIP Grade - ab1759 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab1759 staining Histone H2B (acetyl) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1759, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2B (acetyl K11) antibody - ab40975 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2B (acetyl K11) antibody (ab40975) at 1 µg/mlLane 1 : Calf Thymus Histone Preparation Nuclear Lysate Lane 2 : Histone H2B Human recombinantLane 3 : Histone H3.1 Human recombinantLane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H2B (acetyl K11) peptide (ab41888) at 1 µg/mlLane 5 : Histone H2B Human recombinant with Human Histone H2B (acetyl K11) peptide (ab41888) at 1 µg/mlLane 6 : Histone H3.1 Human recombinant with Human Histone H2B (acetyl K11) peptide (ab41888) at 1 µg/mlLane 7 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H2B peptide (ab35791) at 1 µg/mlLane 8 : Histone H2B Human recombinant with Human Histone H2B peptide (ab35791) at 1 µg/mlLane 9 : Histone H3.1 Human recombinant with Human Histone H2B peptide (ab35791) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-Histone H2B (acetyl K12) antibody - ab61228 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.

Anti-Histone H2B (acetyl K12) antibody [EP858Y] - ab40883 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP858Y
ab40883 staining Histone H2B (acetyl K12) in human differentiated haematopoietic stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/500) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview

Anti-Histone H2B (acetyl K120) antibody - ab176430 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab176430 staining Histone H2B (acetyl K120) in HeLa cells. The cells were fixed with 4% PFA (10min), permabilised with 0.1%Triton (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab176430 at 1μg/ml and ab195889 at 2µg/ml (shown in pseudo color red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ab62335 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP955Y
All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilutionLane 1 : HeLa cell lysate (untreated)Lane 2 : HeLa cell lysate (treated with TSA)Lysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP conjugate at 1/2000 dilution

Anti-Histone H2B (acetyl K16) antibody [EPR17598] - ab177427 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17598
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2B (acetyl K16) with ab177427 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Acetylation level increased after treatment with Trichostatin A (500 ng/ml) for 4 hours.The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab177427 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Anti-Histone H2B (acetyl K20) antibody - ab175361 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2B (acetyl K20) antibody (ab175361) at 1 µg/mlLane 1 : HeLa Sodium Butyrate-treated 0uM Control (Cell acid extract)Lane 2 : HeLa Sodium Butyrate-treated 10uM Control (Cell acid extract)Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : Calf Thymus Histone Preparation Nuclear LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade - ab52988 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP859Y
All lanes : Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade (ab52988) at 1/50000 dilutionLane 1 : C6 cell lysate, untreatedLane 2 : C6 cell lysate, treated with TSALysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Histone H2B (acetyl K20) antibody [EPR859] - ChIP Grade - ab177430 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR859
All lanes : Anti-Histone H2B (acetyl K20) antibody [EPR859] - ChIP Grade (ab177430) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysatesLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) untreated whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000

Anti-Histone H2B (acetyl K24) antibody - ab176429 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab176429 staining Histone H2B (acetyl K24) in HeLa cells. The cells were fixed with 4% PFA (10min), permabilised with 0.1%Triton (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab176429 at 1μg/ml and ab195889 at 2µg/ml (shown in pseudo color red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Anti-Histone H2B (acetyl K24) antibody [EPR17604] - ab177434 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR17604
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, treated with Trichostatin A (500 ng/ml) for 4 hours or non-treated, labeling Histone H2B (acetyl K24) with ab177434 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Acetylation level increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab177434 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa

Anti-Histone H2B (acetyl K5) antibody - ab61227 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H2B (acetyl K5) antibody (ab61227) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.

Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade - ab40886 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP857Y
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.    

Anti-Histone H2B (acetyl K5) antibody - ChIP Grade - ab175046 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab175046 staining Histone H2B (acetyl K5) in HeLa cells. The cells were incubated with 10mM Sodium butyrate (ab120948) for 6 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab175046 at 0.5µg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Anti-HIST2H2BF Antibody (aa14-42, FITC) - LS-C236270 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (aa14-42, Biotin) - LS-C236269 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (aa14-42, AP) - LS-C236267 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (aa13-42) - LS-C321980 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (aa14-42, PE) - LS-C236271 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (aa14-42, APC) - LS-C236268 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (aa14-42, Azide-free, HRP) - LS-C249199 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIST2H2BF Antibody (N-Terminus) - LS-C111846 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HIST2H2BF antibody LS-C111846 Western blot of Fetal Small Intestine lysate.

Anti-HIST2H2BF Antibody (aa14-42) - LS-C166903 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
HIST2H2BF Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The HIST2H2BF antibody detected the HIST2H2BF protein (arrow).
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