Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade

Name	Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade
Supplier	Abcam
Catalog	ab52988
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP859Y
Applications	WB IP FC IHC-P ICC/IF ICC/IF ChIP
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Histone H2B aa 1-100 (acetyl K20)
Description	Rabbit Monoclonal
Gene	HIST2H2BF
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade (ab52988) at 1/50000 dilutionLane 1 : C6 cell lysate, untreatedLane 2 : C6 cell lysate, treated with TSALysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

ab52988 at 1/250-1/500 dilution staining Histone H2B in human cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.

ICC/IF image of ab52988 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52988, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HeLa cells stained with ab52988 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52988, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.