Anti-Histone H2B (acetyl K24) antibody [EPR17604]

• Supplier: Abcam
• Catalog: ab177434
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17604
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H2B aa 1-100 (acetyl K24)
• Description: Rabbit Monoclonal
• Gene: HIST2H2BF
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, treated with Trichostatin A (500 ng/ml) for 4 hours or non-treated, labeling Histone H2B (acetyl K24) with ab177434 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Acetylation level increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab177434 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa

All lanes : Anti-Histone H2B (acetyl K24) antibody [EPR17604] (ab177434) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysatesLane 2 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution