Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab12181
• Prices: $392.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ChIP WB
• Species Reactivities: Mouse, Chicken, Human, Drosophila, Rat, Bovine, Xenopus, C. elegans, Broad species
• Antigen: A synthetic, acetylated and phosphorylated (acetyl K9) & (phospho S10) histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3 conjugated to KLH
• Description: Rabbit Polyclonal
• Gene: CELE_F58A4.3
• Conjugate: Unconjugated

Product images

This image was kindly supplied as part of the review submitted by Monika Harrer.

All lanes : Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)Lane 1 : Whole cell extract of NIH3T3 cellsLane 2 : Whole cell extract of NIH3T3 cells with [Ac-Lys9, pSer10] Histone H3 peptide Lane 3 : Whole cell extract of NIH3T3 cells with [non-Ac, non-phos] Histone H3 peptideLane 4 : Whole cell extract of NIH3T3 cells with [Ac-Lys9] Histone H3 peptide Lane 5 : Whole cell extract of NIH3T3 cells with [pSer10] Histone H3 peptide SecondaryAlkaline phosphatase conjugated Goat anti-Rabbit developed with a colorimetric substrateObserved band size : 17 kDa (why is the actual band size different from the predicted?)

Product References

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