CELE_F58A4.3

Gene Symbol CELE_F58A4.3
Entrez Gene 176359
Alt Symbol -
Species C. elegans
Gene Type protein-coding
Description HCP-3
Other Description -
Swissprots P34470
Accessions CAA80160 P34470 NM_066727 NP_499128
Function Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Not required for chromosome segregation during meiosis. {ECO:0000269|PubMed:10524621, ECO:0000269|PubMed:11402065, ECO:0000269|PubMed:14522947, ECO:0000269|PubMed:16273096}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.

Anti-Histone H3 (acetyl K23) antibody - ab47813 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (acetyl K23) antibody (ab47813) at 1 µg/mlLane 1 : HeLa Histone Preparation Nuclear Lysate - ButyratedLane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 peptide (ab17163) at 0.5 µg/mlLane 3 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - acetyl K36 at 0.5 µg/mlLane 4 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K9) peptide (ab16635) at 0.5 µg/mlLane 5 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - acetyl K14 at 0.5 µg/mlLane 6 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K18) peptide (ab24003) at 0.5 µg/mlLane 7 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K27) peptide (ab24404) at 0.5 µg/mlLane 8 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K23) peptide (ab48359) at 0.5 µg/mlLysates/proteins at 2.5 µg per lan

Anti-Histone H3 (acetyl K9, phospho S10) antibody [APH3-64] - ab12180 from Abcam

Prices $372.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone APH3-64

Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade - ab12181 from Abcam

Prices $392.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
This image was kindly supplied as part of the review submitted by Monika Harrer.

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade - ab5103 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade (ab5103) at 1/1000 dilution + Fruit fly (Drosophila melanogaster) tissue lysate - nuclear at 5 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (Alexa Fluor® 647) - ab196698 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 14955
ab196698 staining Histone H3 (phospho S10) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1 %PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196698 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade - ab14955 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 14955
IHC image of ab14955 staining Histone H3 (phospho S10) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14955, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade (Alexa Fluor® 488) - ab197502 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 14955
ab197502 staining Histone H3 (phospho S10) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197502 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Anti-Histone H3 (phospho S10) antibody - ChIP Grade - ab5176 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab5176 staining Histone H3 (phospho S10) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5176, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (phospho T11) antibody - ChIP Grade - ab5168 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (phospho T11) antibody - ChIP Grade (ab5168) at 1/500 dilution + Calf thymus histone lysateSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilutionPerformed under reducing conditions.

Anti-Histone H3 antibody - ab18521 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Histone H3 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18521.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 17kDa; Histone H3

Anti-Histone H3 antibody - ChIP Grade - ab12079 from Abcam

Prices $400.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
IHC image of Histone H3 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12079, 5µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 antibody - ChIP Grade - ab85869 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab85869 staining Histone H3 in human colon formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85869, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade - ab1791 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 3 : Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr)Lane 4 : S.cerevisiae (Y190) Whole Cell Lysate Lane 5 : S.pombe Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

hcp-3 Antibody - 29540002 from Novus Biologicals

Prices $499.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: hcp-3 Antibody [29540002] - This image is specific to animal number Q0806 Dilution 1:500

Goat anti-Caenorhabditis hcp-3 Polyclonal antibody - CPBT-53638GC from Creative Diagnostics

Host Goat
Clonality Polyclonal

Rabbit anti-hcp-3 Polyclonal antibody - DPAB-CL016 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

HCP-3, Polyclonal Antibody - MBS417814 from MyBioSource

Prices $280.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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