Anti-Histone H3 (citrulline R26) antibody - ChIP Grade
| Name | Anti-Histone H3 (citrulline R26) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab19847 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ChIP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3 |
| Description | Rabbit Polyclonal |
| Gene | H3F3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Histone H3 (citrulline R26) antibody - ChIP Grade (ab19847) at 1/1000 dilutionLane 1 : MCF7 acid-extracted histonesLane 2 : MCF7 acid-extracted histones, treated with recombinant PADI2SecondaryAnti rabbit HRP conjugated at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab19847 gave a positive result in ELISA against the immunising peptide (ab20631) and a negative result using the non-modified equivalent peptide (ab2623). This indicates that ab19847 is specifically recognising Histone H3 citrulline R26.Green and Orange lines: ELISA against immunising peptide (ab20631)Red and Mauve lines: ELISA against non-modified peptide (ab2623)
ab19847 gave a positive result in ELISA against the immunising peptide (ab20631) and a negative result using the non-modified equivalent peptide (ab2623). This indicates that ab19847 is specifically recognising Histone H3 citrulline R26.Green and Orange lines: ELISA against immunising peptide (ab20631)Red and Mauve lines: ELISA against non-modified peptide (ab2623)
ab19847 gave a positive result in ELISA against the immunising peptide (ab20631) and a negative result using the non-modified equivalent peptide (ab2623). This indicates that ab19847 is specifically recognising Histone H3 citrulline R26.Green and Orange lines: ELISA against immunising peptide (ab20631)Red and Mauve lines: ELISA against non-modified peptide (ab2623)
ChIP of H3Cit26 at the TFF1 gene promoter of MCF-7 cells with ab19847. As expected H3Cit26 was enriched at the TFF1 promoter of cells treated with Estrogen for 45' (E) compared to cells treated with Ethanol as a negative control (U).
MCF-7 breast cancer cells were cultured for 3 days in DMEM phenol red-free medium supplemented with 10% charcoal-dextran-treated calf serum and treated with Estrogen for 45 min (positive control) or EtOH (negative control).The immunoprecipitated DNA was quantified by real time PCR (SYBR). Primers are located in ERE region (-273bp,-198bp relative to the TSS) of TFF1 gene.See Abreview
Product References
Targeted H3R26 deimination specifically facilitates estrogen receptor binding by - Targeted H3R26 deimination specifically facilitates estrogen receptor binding by
Guertin MJ, Zhang X, Anguish L, Kim S, Varticovski L, Lis JT, Hager GL, Coonrod SA. PLoS Genet. 2014 Sep 11;10(9):e1004613.
Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination - Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination
Zhang X, Bolt M, Guertin MJ, Chen W, Zhang S, Cherrington BD, Slade DJ, Dreyton CJ, Subramanian V, Bicker KL, Thompson PR, Mancini MA, Lis JT, Coonrod SA. Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13331-6. doi:
Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of - Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of
Cherrington BD, Morency E, Struble AM, Coonrod SA, Wakshlag JJ. PLoS One. 2010 Jul 26;5(7):e11768.