H3F3A

Gene Symbol H3F3A
Entrez Gene 3020
Alt Symbol H3.3A, H3F3
Species Human
Gene Type protein-coding
Description H3 histone, family 3A
Other Description histone H3.3
Accessions CAA29288 EAW69770 EAW69771 EAW69772 EAW69773 P84243 AK293541 BAG57022 AK311905 BAG34846 BC013857 BC029405 AAH29405 BC038989 AAH38989 BC081561 AAH81561 BC095447 AAH95447 BM760856 CA310975 D28384 BAA05750 DQ892615 ABM83541 DQ895855 ABM86781 M11353 AAA52654 M11354 AAA52653 NM_002107 NP_002098
Top Pathways Systemic lupus erythematosus, Alcoholism, Transcriptional misregulation in cancer

Anti-Histone H3 (acetyl K18) antibody - ab189884 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (acetyl K18) antibody (ab189884) at 1/500 dilutionLane 1 : Hela cells treated with TSA 400 nM 24hLane 2 : Hela cells treated with TSA 400 nM 24h + immunizing peptide

Anti-Histone H3 (acetyl K9) antibody [Y28] - ab32129 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y28
IHC image of ab32129 staining Histone H3 (acetyl K9) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32129, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [7C10] - ab80256 from Abcam

Prices $387.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7C10
ab80256 staining Histone H3 (citrulline 2 + 8 + 17) in human sputum cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde and permeabilized using saponin. Samples were then blocked and incubated with ab80256 at a 1/100 dilution. The secondary used was a goat anti-mouse IgG monoclonal conjugated to Alexa Fluor 546, 1/500 dilution. Counterstained with Hoechst.See Abreview

Anti-Histone H3 (citrulline R2) antibody - ab174992 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab174992 staining Histone H3 (citrulline R2) in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab174992 at 5µg/ml and ab7291 (anti beta Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat anti-rabbit AlexaFluor®488 secondary antibody (ab150081) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Anti-Histone H3 (citrulline R2) antibody [EPR17703] - ab176843 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17703
All lanes : Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilutionLane 1 : MCF7 (Human breast adenocarcinoma cell line) cells transfected with vector controlLane 2 : MCF7 (Human breast adenocarcinoma cell line) cells transfected with PADI4SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H3 (citrulline R26) antibody - ChIP Grade - ab19847 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (citrulline R26) antibody - ChIP Grade (ab19847) at 1/1000 dilutionLane 1 : MCF7 acid-extracted histonesLane 2 : MCF7 acid-extracted histones, treated with recombinant PADI2SecondaryAnti rabbit HRP conjugated at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (phospho S10 + S28) antibody - ab46986 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho S10 + S28) antibody (ab46986) at 1/5000 dilutionLane 1 : Acid extract of HeLa cells, no treatment Lane 2 : Acid extract of HeLa cells, treatment with colcemid.

Anti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade - ab177218 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17246
All lanes : Anti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade (ab177218) at 1/200 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 1.5µg/ml Colcemid for 12 hours whole cell lysatesLane 2 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H3 (phospho S28) antibody - ab183767 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho S28) antibody (ab183767) at 1/1000 dilutionLane 1 : HeLa whole cell lysate (untreated)Lane 2 : HeLa whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr)Lysates/proteins at 30 µg per lane.

Anti-Histone H3 (phospho S28) antibody - ab53763 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho S28) antibody (ab53763) at 1/500 dilutionLane 1 : HuvEc cell extract, treated with Serum (20%, 30mins)Lane 2 : HuvEc cell extract, treated with Serum (20%, 30mins) with immunising peptide

Anti-Histone H3 (phospho S28) antibody [117C826] - ab13924 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 117C826

Anti-Histone H3 (phospho S28) antibody [E191] - ab32388 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E191
Dot blot performed using ab32388 at a dilution of 1/100. Lane 1 - Unmodified H3 peptide. Lane 2 - H3S28ph peptide. Lane 3 - H3.3S28ph peptide. Lane 4 - H3.3S31ph peptide. A HRP conjugated goat anti-rabbit (H+L) was used as the secondary antibody at a dilution of 1/2500. The exposure time was 3 minutes and the dilution and blocking buffer used were 5% NFDM/TBST.

Anti-Histone H3 (phospho S28) antibody [EP1874Y] - ab76312 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1874Y
All lanes : Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312) at 1/5000 dilutionLane 1 : HeLa cell lysates untreatedLane 2 : HeLa cell lysates treated with FBS + calyculin ALysates/proteins at 10 µg per lane.

Anti-Histone H3 (phospho T3) antibody - ab53762 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho T3) antibody (ab53762) at 1/500 dilutionLane 1 : HuvEc cell extracts, treated with Serum (20%, 30mins)Lane 2 : HuvEc cell extracts, treated with Serum (20%, 30mins) with immunising peptide

Anti-Histone H3 (phospho T3) antibody [EP1702Y] - ab78351 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1702Y
All lanes : Anti-Histone H3 (phospho T3) antibody [EP1702Y] (ab78351) at 1/500000 dilutionLane 1 : HeLa cell lysatesLane 2 : HeLa cell lysates treated with FBS + Calyculin A.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Histone H3 (phospho T6) antibody - ChIP Grade - ab14102 from Abcam

Prices $400.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (phospho T6) antibody - ChIP Grade (ab14102) at 1 µg + HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3.3 (phospho S31) antibody - ab62207 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62207 staining Histone H3.3 (phospho S31) in HeLa by Immunocytochemistry/ Immunofluorescence. The image is shown with and without the blocking phosphopeptide.

Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - ChIP Grade - ab92628 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1873
All lanes : Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - ChIP Grade (ab92628) at 1/1000 dilutionLane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate treated with Calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade - ab176840 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17899
All lanes : Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (ab176840) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H3.3 antibody - ChIP Grade - ab62642 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells, using ab62642 at a 1/500 dilution. Left image untreated. Right image treated with peptide.

Anti-Histone H3 antibody - ab61251 from Abcam

Prices $397.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61251 at 1/50 - 1/100 dilution staining Histone H3 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-Histone H3 antibody - ab39655 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 antibody (ab39655) at 1/500 dilutionLane 1 : COLO205 cell extracts 5-30µg/lane with blocking peptide at 1 µg/mlLane 2 : COLO205 cell extracts 5-30µg/lane

Anti-Histone H3 antibody - ab61252 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61252 staining Histone H3 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab61252 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat anti-rabbit AlexaFluor®488 secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H3 staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.

Anti-Histone H3 antibody - ab187958 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Histone H3 antibody - ab62706 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab62706 staining Histone H3 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62706, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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