Anti-Histone H3 (di methyl K36) antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab9049
• Prices: $404.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB ChIP
• Species Reactivities: Mouse, Bovine, Human, Pig, Yeast, Arabidopsis thaliana, C. elegans, Drosophila, Zebrafish, Dictyostelium discoideum, Mammalian
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3
• Blocking Peptide: Human Histone H3 (di methyl K36) peptide
• Description: Rabbit Polyclonal
• Gene: HIST3H3
• Conjugate: Unconjugated

Product images

IHC image of ab9049 staining Histone H3 (di methyl K36) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9049, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ab9049 staining Histon H3 (di methyl K36) in Mouse Neuro-2a neuroblastoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

All lanes : Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) at 1 µg/mlLane 1 : Calf Thymus Histone Preparation Nuclear LysateLane 2 : Calf Thymus Histone Preparation Nuclear Lysate with Histone H3 peptide (ab41430) at 0.5 µg/mlLane 3 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K36) peptide (ab1783) at 0.5 µg/mlLane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K36) peptide (ab1784) at 0.5 µg/mlLane 5 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K36) peptide (ab1785) at 0.5 µg/mlLane 6 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 0.5 µg/mlLane 7 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 0.5 µg/mlLane 8 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 0.5 µg/mlLane 9 : Calf Thy

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab9049(blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and RPL30 (active) promoters and over the g-Actin gene (active). Schematic diagram of the g-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

ab9049 staining Histone H3 (di methyl K36) in C elegans intestinal cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde and permeabilized using sperm salts buffer, Triton X-100. Samples were then incubated with ab9049 at a 1/200 for 16 hours at 22°C. A FITC conjugated goat polyclonal was used as the secondary at a 1/100 dilution. Counterstained with DAPI.See Abreview

All lanes : Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) at 1/1400 dilutionLane 1 : Whole cell lysate prepared from Drosophila BG3 tissue cellsLane 2 : Whole cell lysate prepared from Drosophila BG3 tissue cellsLysates/proteins at 50000 cells per lane.SecondaryDonkey anti-rabbit polyclonal conjugated to HRP at 1/20000 dilutiondeveloped using the ECL technique

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