HIST3H3

Gene Symbol HIST3H3
Entrez Gene 8290
Alt Symbol H3.4, H3/g, H3FT, H3t
Species Human
Gene Type protein-coding
Description histone cluster 3, H3
Other Description H3 histone family, member T|H3/t|histone 3, H3|histone H3.1t
Swissprots Q16695 Q6FGU4 B2R5K3
Accessions AAN39284 CAA90020 EAW69877 Q16695 AK312217 BAG35150 BC069079 AAH69079 BC101837 AAI01838 BC101839 AAI01840 CR542013 CAG46810 DQ587912 NM_003493 NP_003484
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus. Chromosome.
Tissue Specificity Expressed in testicular cells.
Top Pathways Systemic lupus erythematosus, Alcoholism, Transcriptional misregulation in cancer

Anti-HIST3H3 antibody - ab180727 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HIST3H3 antibody (ab180727) at 1/500 dilutionLane 1 : Hela cell lysateLane 2 : H3 protein expressed in E.coli

Anti-Histone H3 (asymmetric di methyl R17) antibody - ab194707 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (asymmetric di methyl R17) antibody (ab194707) at 1/500 dilutionLane 1 : Extracts from HeLa cellsLane 2 : H3 protein expressed in E. coli

Anti-Histone H3 (asymmetric di methyl R17) antibody - ChIP Grade - ab8284 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All batches of ab8284 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - asymmetric di methyl R17 peptide (ab16935), indicating that this antibody specifically recognises the Histone H3 - asymmetric di methyl R17 modification.ab16935 - Histone H3 - asymmetric di methyl R17ab32948 - Histone H3 - symmetric di methyl R17ab14663 - Histone H3 - unmodified

Anti-Histone H3 (asymmetric di methyl R17, symmetric di methyl R17) antibody - ab174566 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab174566 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - asymmetric di methyl R17 peptide (ab16935) and Histone H3 - symmetric di methyl R17 peptide (ab32948).ab14663 - Histone H3 - unmodifiedab16935 - Histone H3 - asymmetric di methyl R71ab32948 - Histone H3 - symmetric di methyl R17

Anti-Histone H3 (asymmetric di methyl R2) antibody - ab194706 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706) at 1/500 dilutionLane 1 : Extracts from HeLa cellsLane 2 : H3 protein expressed in E. coli

Anti-Histone H3 (asymmetric di methyl R2) antibody - ab80075 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] - ab176845 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17705
All lanes : Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (ab176845) at 1/200 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade - ab175007 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade (ab175007) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 2 : HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µgLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µgLane 4 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µgSecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Anti-Histone H3 (asymmetric di methyl R8) antibody - ab127163 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab127163 staining Histone H3 (asymmetric di methyl R8) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127163, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (asymmetric di methyl R8) antibody - ab194702 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (asymmetric di methyl R8) antibody (ab194702) at 1/500 dilutionLane 1 : Extracts from HeLa cellsLane 2 : H3 protein expressed in E. coli

Anti-Histone H3 (di methyl K27) antibody - ab194690 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of 293T cells labeling Histone H3 with ab194690 at 1/50 dilution (red). Blue: DAPI for nuclear staining.

Anti-Histone H3 (di methyl K27) antibody - ab110472 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation : The nuclear extract derived from 3T3 were immunoprecipitated by 4 &microg of ab110472. Western Blot: Rabbit anti Dimethyl Histone 3 (K9), antibody at 1/1000

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade - ab24684 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab24684) at 1 µg/mlLane 1 : Calf thymus histone lysateLane 2 : Calf thymus histone lysate with Human Histone H3 peptide (ab17163) at 0.5 µg/mlLane 3 : Calf thymus histone lysate with Human Histone H3 (mono methyl K4) peptide (ab1340) at 0.5 µg/mlLane 4 : Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (ab7768) at 0.5 µg/mlLane 5 : Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (ab1342) at 0.5 µg/mlLane 6 : Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 0.5 µg/mlLane 7 : Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 0.5 µg/mlLane 8 : Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 0.5 µg/mlLane 9 : Calf thymus histone lysate with Human Histone H3 (mono methyl K27) peptide (ab1780) at 0.5 µg/mlLane 10 : Calf thymus histone lysate with Human Histone H3 (di methy

Anti-Histone H3 (di methyl K36) antibody [RM141] - ab200536 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Monoclonal
Clone RM141
All lanes : Anti-Histone H3 (di methyl K36) antibody [RM141] (ab200536) at 0.5 µg/mlLane 1 : recombinant Histone H3.3Lane 2 : Acid extracts of HeLa cells

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade - ab9049 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab9049 staining Histone H3 (di methyl K36) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9049, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (di methyl K4) antibody - ab194678 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (di methyl K4) antibody (ab194678) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : Histone H3 protein expressed in E.coli

Anti-Histone H3 (di methyl K4) antibody - ab131711 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade - ab32356 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y47
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µl of ab32356 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H3 (di methyl K4) antibody - ChIP Grade - ab7766 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab7766 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H3 (di methyl K4) antibody - ChIP Grade - ab11946 from Abcam

Prices $398.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 2µg of  ab11946 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H3 (di methyl K4) antibody - ChIP Grade - ab59563 from Abcam

Prices $378.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
ab59563 was used to immunoprecipitate histone H3 di methyl K4 from mono-nucleosomes isolated from mature chicken oviduct. Lanes 1 - 3 Coomassie stain Lanes 4 - 6 ab8580 staining to demonstrate IP specificity Lanes 1 & 4 - Input Lanes 2 & 5 - Unbound Lanes 3 & 6 - Bound

Anti-Histone H3 (di methyl K79) antibody - ab194689 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Chromatin immunoprecipitation analysis of γ - actin gene from 293 cell line, using ab194689 and rabbit IgG. P1, P2 and P3 were probes located on γ - actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checkedby quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3 (di methyl K79) antibody [EPR17467] - ab177184 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17467
All lanes : Anti-Histone H3 (di methyl K79) antibody [EPR17467] (ab177184) at 1/2500 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H3 (di methyl K79) antibody - ChIP Grade - ab3594 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab3594 staining Histone H3 (di methyl K79) in human differentiated haematopoietic stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/800) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat polyclonal anti-rabbit IgG (1/1500) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview

Anti-Histone H3 (di methyl K9) antibody - ab194680 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of 293T cells labeling Histone H3 (di methyl K9) with ab194680 at 1/50 dilution (red). Blue: DAPI for nuclear staining.
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