Chromatin immunoprecipitation analysis of γ - actin gene from 293 cell line, using ab194689 and rabbit IgG. P1, P2 and P3 were probes located on γ - actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checkedby quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunofluorescent analysis of 293T cells labeling Histone H3 with ab194689 at 1/50 dilution (red). Blue: DAPI for nuclear staining.
All lanes : Anti-Histone H3 (di methyl K79) antibody (ab194689) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : Histone H3 protein expressed in E.coliLane 3 : HeLa cell extractLane 4 : Histone H3 protein expressed in E.coli