Anti-Histone H3 (phospho S10) antibody - ChIP Grade
| Name | Anti-Histone H3 (phospho S10) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab5176 |
| Prices | $404.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P IHC IHC-F FC ChIP IHC-F |
| Species Reactivities | Mouse, Rat, Hamster, Bovine, Human, Xenopus, Drosophila, Deer, Zebrafish, Monkey, Fish, Yeast, C. elegans, S. pombe, Neurospora crassa |
| Antigen | Synthetic peptide corresponding to Human Histone H3 aa 1-100 (phospho S10) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | CELE_F58A4.3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab5176 staining Histone H3 (phospho S10) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5176, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image was kindly supplied by Prof Bryan Turner, University of Birmingham. Female Human Lymphoblastoid cells were incubated with ab5176.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
SKN cells stained with ab5176 (green) at a dilution of 1/100. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab5176. The DNA is stained with DAPI (blue). 100x magnification.
ab5176 at a 1/100 dilution staining Drosophila melanogaster (wild type) polytene chromosomes by ICC/IF. The cells were formaldehyde fixed and blocked with 1% BSA prior to incubation with the antibody for 12 hours. Bound antibody was detected using a Cy3 conjugated goat anti-rabbit antibody.See Abreview
ab5176 (1/1000) staining a population of human HeLa cells positive for Histone H3 (phospho S10). Cells were trypsizined, pelleted and fixed in ice cold Ethanol. Debris was eliminated and FL2-A/FL2-W was used to eliminate clumping cells. For further experimental details please refer to abreview folder.See Abreview
IHC-Wholemount image of Histone H3 (phospho S10) ab5176 on zebrafish jaw. 5 day old zebrafish were permeabilized with proteinase K (10ug/ml, 50min, 37degrees), prior to staining. Staining visible in mitotic cells. Image shows 2 cells labelled with H3 phospho S10 antibody (green), nuclei are labelled with DAPI (blue).See Abreview
ab14404 staining Histone H3 (phospho S10) - Mitosis Marker in Cyanidioschyzon merolae cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% BSA) for 1 hour at 37°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.See Abreview
Product References
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Thomas C, Strutt D. Dev Biol. 2014 Jan 1;385(1):107-21.
Diversity-oriented synthesis as a tool for identifying new modulators of mitosis. - Diversity-oriented synthesis as a tool for identifying new modulators of mitosis.
Ibbeson BM, Laraia L, Alza E, O' Connor CJ, Tan YS, Davies HM, McKenzie G, Venkitaraman AR, Spring DR. Nat Commun. 2014;5:3155.
Mutations in Alstrom protein impair terminal differentiation of cardiomyocytes. - Mutations in Alstrom protein impair terminal differentiation of cardiomyocytes.
Shenje LT, Andersen P, Halushka MK, Lui C, Fernandez L, Collin GB, Amat-Alarcon N, Meschino W, Cutz E, Chang K, Yonescu R, Batista DA, Chen Y, Chelko S, Crosson JE, Scheel J, Vricella L, Craig BD, Marosy BA, Mohr DW, Hetrick KN, Romm JM, Scott AF, Valle D, Naggert JK, Kwon C, Doheny KF, Judge DP. Nat Commun. 2014 Mar 4;5:3416.
The thrombospondin-1 receptor CD36 is an important mediator of ovarian - The thrombospondin-1 receptor CD36 is an important mediator of ovarian
Osz K, Ross M, Petrik J. Reprod Biol Endocrinol. 2014 Mar 14;12:21.
Requirement for Parp-1 and DNA ligases 1 or 3 but not of Xrcc1 in chromosomal - Requirement for Parp-1 and DNA ligases 1 or 3 but not of Xrcc1 in chromosomal
Soni A, Siemann M, Grabos M, Murmann T, Pantelias GE, Iliakis G. Nucleic Acids Res. 2014 Jun;42(10):6380-92.
Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium - Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium
Tomas J, Reygner J, Mayeur C, Ducroc R, Bouet S, Bridonneau C, Cavin JB, Thomas M, Langella P, Cherbuy C. ISME J. 2015 Jan;9(1):46-58.
Nestin downregulation in rat vascular smooth muscle cells represents an early - Nestin downregulation in rat vascular smooth muscle cells represents an early
Tardif K, Hertig V, Dumais C, Villeneuve L, Perrault L, Tanguay JF, Calderone A. Cardiovasc Diabetol. 2014 Aug 21;13:119.
Atm deletion with dual recombinase technology preferentially radiosensitizes - Atm deletion with dual recombinase technology preferentially radiosensitizes
Moding EJ, Lee CL, Castle KD, Oh P, Mao L, Zha S, Min HD, Ma Y, Das S, Kirsch DG. J Clin Invest. 2014 Aug;124(8):3325-38.
Aurora-A inhibition offers a novel therapy effective against intracranial - Aurora-A inhibition offers a novel therapy effective against intracranial
Van Brocklyn JR, Wojton J, Meisen WH, Kellough DA, Ecsedy JA, Kaur B, Lehman NL. Cancer Res. 2014 Oct 1;74(19):5364-70.
IGF-1R inhibition enhances radiosensitivity and delays double-strand break repair - IGF-1R inhibition enhances radiosensitivity and delays double-strand break repair
Chitnis MM, Lodhia KA, Aleksic T, Gao S, Protheroe AS, Macaulay VM. Oncogene. 2014 Nov 6;33(45):5262-73.