Anti-Histone H3 (phospho T6) antibody - ChIP Grade
| Name | Anti-Histone H3 (phospho T6) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab14102 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP IHC-P WB ICC/IF ICC/IF Array |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human Histone H3 aa 1-100 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Histone H3 (phospho T6) peptide |
| Description | Rabbit Polyclonal |
| Gene | H3F3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Histone H3 (phospho T6) antibody - ChIP Grade (ab14102) at 1 µg + HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All batches of ab14102 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - phospho T6 peptide (ab14103), indicating that this antibody specifically recognises the Histone H3 - phospho T6 modification.ab7228 - Histone H3 unmodifiedab17578 - Histone H3 - phospho T3ab14103 - Histone H3 - phospho T6ab24444 - Histone H3 - phospho T11ab11477 - Histone H3 - phospho S10ab32876 - Histone H3 - citrulline 2 + 8 + 17
ab14102 staining Histone H3 in HeLa cells, by ICC/IF, in conjunction with a secondary goat anti-rabbit antibody conjugated to Cy3® (green). Cells counterstained with DAPI to show the nucleus (red).See Abreview
Product References
Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone - Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone
Metzger E, Imhof A, Patel D, Kahl P, Hoffmeyer K, Friedrichs N, Muller JM, Greschik H, Kirfel J, Ji S, Kunowska N, Beisenherz-Huss C, Gunther T, Buettner R, Schule R. Nature. 2010 Apr 1;464(7289):792-6.
Phosphorylation of histone H3 Thr-45 is linked to apoptosis. - Phosphorylation of histone H3 Thr-45 is linked to apoptosis.
Hurd PJ, Bannister AJ, Halls K, Dawson MA, Vermeulen M, Olsen JV, Ismail H, Somers J, Mann M, Owen-Hughes T, Gout I, Kouzarides T. J Biol Chem. 2009 Jun 12;284(24):16575-83.
Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and - Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and
Tjeertes JV, Miller KM, Jackson SP. EMBO J. 2009 Jul 8;28(13):1878-89.