Bmi1 (D20B7) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 6964
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D20B7
• Applications: WB IP IHC-P ICC/IF FC ChIP
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Bmi1 protein.
• Description: Rabbit Monoclonal
• Gene: BMI1

Product images

Western blot analysis of extracts from various cell lines using Bmi1 (D20B7) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bmi1 (D20B7) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoma using Bmi1 (D20B7) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of COS-7 cells using Bmi1 (D20B7) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and either 10 µl of Bmi1 (D20B7) XP ® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human HoxA1 Intron 1 Primers #7707, SimpleChIP ® Human HoxA2 Promoter Primers #5517, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).