Immunohistochemical analysis of frozen cytospin preparations of HEK293T cells expressing HJURP (left and right panel) or A-MYB (central panel) as negative control. The left and central panels were incubated with ab175577, labeling HJURP. The right panel was incubated with an antibody against the expression vector itself (positive control).
All lanes : Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470) at 1/100 dilutionLane 1 : Human kidney lysateLane 2 : Human colon lysateLane 3 : K562 cell lysateLane 4 : Daudi cell lysateLane 5 : Raji cell lysateLysates/proteins at 100 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil, tonsil epithelium, thymus and testicle tissues, labeling HJURP using ab175577 at a 1/400 dilution.
Immunofluorescence analysis of Human tonsil tissue, labeling HJURP using ab175577 at a 1/200 dilution (green), followed by washing, antifading and DAPI staining (blue).