Anti-HLTF antibody - ChIP Grade
| Name | Anti-HLTF antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab17984 |
| Prices | $380.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP WB IP |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan, Platypus |
| Antigen | Synthetic peptide |
| Description | Rabbit Polyclonal |
| Gene | HLTF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![SMARCA3 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to HLTF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17984.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 113kDa; SMARCA3, non specific bands - 50kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/2793_ab17984-203481-IPVI001ab179843m.jpg)
SMARCA3 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to HLTF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17984.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 113kDa; SMARCA3, non specific bands - 50kDa: We are unsure as to the identity of this extra band.
ab17984 in ChIP of rabbit endometrium nuclear cell lysate. In vivo binding of SMARCA3 (RUSH) to DNA and Egr-1/c-Rel in the rabbit RUSH promoter was confirmed by PCR amplification of products from ChIP assays. Lane 1 FX174/HaeIII size markers. Lane 2 Single 166-bp amplicons of the SMARCA3 (RUSH) site resulting from ChIP with antibodies to SMARCA3. Lanes 3 Negative ChIP control (IgG) Lane 4 PCR control (water)Lane 5 Single 153-bp amplicons of the Sp1(A) site resulting from ChIP with antibodies to SMARCA3. The SMARCA3 (RUSH) site was identified through antibody-SMARCA3-DNA binding. The Sp1(A) site is an Egr-1/Sp1/3/MAZ/MZF1/c-Rel composite binding site that was identified through antibody-SMARCA3-Egr-1/c-Rel-DNA binding. Cross-linking agent: disuccinimydyl glutarate and formaldehyde (0.75%)See Abreview
Product References
Helicase-like transcription factor (Hltf) regulates G2/M transition, - Helicase-like transcription factor (Hltf) regulates G2/M transition,
Helmer RA, Martinez-Zaguilan R, Dertien JS, Fulford C, Foreman O, Peiris V, Chilton BS. PLoS One. 2013 Nov 20;8(11):e80461.
Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH - Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH
Helmer RA, Panchoo M, Dertien JS, Bhakta SM, Hewetson A, Chilton BS. Mol Cell Endocrinol. 2010 Aug 30;325(1-2):143-9.
Progesterone-dependent deoxyribonucleic acid looping between RUSH/SMARCA3 and - Progesterone-dependent deoxyribonucleic acid looping between RUSH/SMARCA3 and
Hewetson A, Chilton BS. Mol Endocrinol. 2008 Apr;22(4):813-22.