HLTF

Gene Symbol HLTF
Entrez Gene 6596
Alt Symbol HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1
Species Human
Gene Type protein-coding
Description helicase-like transcription factor
Other Description DNA-binding protein/plasminogen activator inhibitor 1 regulator|DNA-binding protein/plasminogen activator inhibitor-1 regulator|RING finger protein 80|SNF2-like 3|SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3|sucrose nonfermenting protein 2-like 3|sucrose nonfermenting-like 3
Swissprots Q14536 Q86YA5 Q7KYJ6 Q92652 Q14527 Q96KM9 D3DNH3 Q16051
Accessions CAD10805 EAW78889 EAW78890 EAW78891 EAW78892 EAW78893 Q14527 AB209052 BAD92289 AI971472 AK291231 BAF83920 BC005260 AAH05260 BC014326 BC015498 AAH15498 BC030976 AAH30976 BC044659 AAH44659 EU446704 ABZ92233 L34673 AAA67436 S64671 AAB27691 XM_005247724 XP_005247781 XM_011513091 XP_011511393 XM_011513092 XP_011511394 XM_011513093 XP_011511395 NM_003071 NP_003062 NM_139048 NP_620636
Function Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. {ECO:0000250, ECO:0000269|PubMed:10391891, ECO:0000269|PubMed:18316726, ECO:0000269|PubMed:18719106, ECO:0000269|PubMed:7876228, ECO:0000269|PubMed:8672239, ECO:0000269|PubMed:9126292}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000269|PubMed:8672239}. Nucleus, nucleolus {ECO:0000250}. Nucleus, nucleoplasm {ECO:0000250}. Note=Nuclear localization is stimulated by progesterone. {ECO:0000250}.
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. {ECO:0000269|PubMed:8672239, ECO:0000269|PubMed:9126292}.

HLTF (G-6) - sc-398357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HLTF (G-6): sc-398357. Western blot analysis of HLTF expression in HeLa (A) and NTERA-2 cl.D1 (B) nuclear extracts.

HLTF (D-15) - sc-27543 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HLTF (Y-20) - sc-27542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HLTF (Y-20): sc-27542. Western blot analysis of HLTF expression in K-562 nuclear extract.

HLTF (H-87) - sc-366563 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HLTF (H-87): sc-366563. Western blot analysis of HLTF expression in K-562 (A), HeLa (B), Jurkat (C) and Ramos (E) nuclear extracts and NTERA-2 cl.D1 whole cell lysate (D).

Anti-HLTF antibody - ab155031 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HLTF antibody (ab155031) at 1/5000 dilution + HeLa whole cell lysate at 30 µg

Anti-HLTF antibody - ab45311 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HLTF antibody - ab3852 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-HLTF antibody - ab2835 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-HLTF antibody [EPR14760] - ab188567 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14760
All lanes : Anti-HLTF antibody [EPR14760] (ab188567) at 1/2000 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-HLTF antibody [EPR14761] - ab183042 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14761
All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilutionLane 1 : HeLa lysateLane 2 : K562 lysateLane 3 : Jurkat lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-HLTF antibody - ChIP Grade - ab17984 from Abcam

Prices $380.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMARCA3 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to HLTF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17984.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 113kDa; SMARCA3, non specific bands - 50kDa: We are unsure as to the identity of this extra band.

Anti-SMARCA3 / HLTF Antibody (aa50-292) - LS-C68165 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA3 / HLTF Antibody - LS-C89130 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA3 / HLTF Antibody (aa900-950) - LS-C287146 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA3 / HLTF Antibody (C-Terminus) - LS-C31506 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-SMARCA3 / HLTF Antibody (N-Terminus) - LS-C31507 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-SMARCA3 / HLTF Antibody (N-Terminus) - LS-C80187 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.

Anti-SMARCA3 / HLTF Antibody (aa409-437) - LS-C166874 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of HIP116A Antibody in HL60 cell line lysates (35 ug/lane). HIP116A (arrow) was detected using the purified antibody.

Anti-SMARCA3 / HLTF Antibody (aa874-887) - LS-C55484 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (0.5 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-SMARCA3 / HLTF Antibody (aa600-700) - LS-C287147 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse SMARCA3 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

HLTF Antibody - NB100-261 from Novus Biologicals

Prices $299.00
Sizes 25 µl
Host Rabbit
Clonality Polyclonal

HLTF Antibody - NB100-1041 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: HLTF Antibody [NB100-1041] (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HLTF Antibody - NBP2-16857 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: HLTF Antibody [NBP2-16857] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:5000.

HLTF Antibody - NBP1-83256 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: HLTF Antibody [NBP1-83256] - Analysis in human cell line HEL.

HLTF Antibody - NB100-280 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: HLTF Antibody [NB100-280] - Nuclear extract (20 ug) from HeLa Cells. Antibody used at 0.5 ug/ml.
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