Anti-HMGA1 antibody [EPR16649]

• Supplier: Abcam
• Catalog: ab202070
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16649
• Applications: FC WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant full length protein within Human HMGA1 aa 1 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: HMGA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilutionLane 1 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysateLane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysateLane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 4 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysateLane 5 : Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysateLane 6 : Human testis lysateLane 7 : Human lung lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling HMGA1 with ab202070 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab202070 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA1 with ab202070 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.