Anti-HMGA1 antibody [EPR7839]

• Supplier: Abcam
• Catalog: ab129153
• Prices: $395.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7839
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HMGA1 (N terminal)
• Description: Rabbit Monoclonal
• Gene: HMGA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 10000 (purified)Lane 1 : HepG2 cell lysateLane 2 : SK-OV-3 cell lysateLane 3 : Caco-2 cell lysateLane 4 : BxPC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution (purified) + Mouse brain tissue lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution (unpurified)Lane 1 : SK-OV-3 cell lysateLane 2 : Caco-2 cell lysateLane 3 : BxPC-3 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling HMGA1 with purified ab129153 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling HMGA1 with unpurified ab129153 at 1/250.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HMGA1 with purified ab129153 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of BxPC-3 cells labelling HMGA1 with unpurified ab129153 at 1/250 dilution.

Flow cytometry analysis of HepG2 cells labelling HMGA1 with purified ab129153 at 1/60 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Overlay histogram showing HepG2 cells stained with unpurified ab129153 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129153, 1/870 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Product References

miR-26a inhibits proliferation and motility in bladder cancer by targeting HMGA1. - miR-26a inhibits proliferation and motility in bladder cancer by targeting HMGA1.

Lin Y, Chen H, Hu Z, Mao Y, Xu X, Zhu Y, Xu X, Wu J, Li S, Mao Q, Zheng X, Xie L. FEBS Lett. 2013 Aug 2;587(15):2467-73.

Expression of high mobility group A proteins in oral leukoplakia. - Expression of high mobility group A proteins in oral leukoplakia.

Larsson L, Jawert F, Magnusson B, Hasseus B, Kjeller G. Anticancer Res. 2013 Oct;33(10):4261-6.