Anti-HMGA1a / HMGA1b antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab4078
• Prices: $398.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ChIP IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Zebrafish, Rat, Chicken, Neurospora crassa, Hamster
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HMGA1a/ HMGA1b
• Description: Rabbit Polyclonal
• Gene: HMGA1
• Conjugate: Unconjugated

Product images

Immunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.A: Hoechst stainB: PhasecontrastC: TexasRed HMG-I/HMG-Y (ab4078)D: HMGA1a-GFPE: Merge

Lanes 1 - 4 : Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078) at 1/1000 dilutionLanes 5 - 8 : Anti-GFPLane 1 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a GFP fusion proteins.Lane 2 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteinsLane 3 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteinsLane 4 : hole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteinsLane 5 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-GFP fusion proteinsLane 6 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteinsLane 7 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteinsLane 8 : Whole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteinsSecondaryAnti-rabbit HRP at 1/10000 di

Chromatin was prepared from the Human Embryonic Stem Cells. The cross-linking (X-ChiP) technique was used; crosslinking was performed for 15 minutes in formaldehyde. The primary antibody was diluted 1/100 in IP dilution buffer and incubated with the sample for 12 hours at 4°C. Histone H3 was used as the positive control, whilst normal rabbit IgG was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR.

IHC image of ab4078 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Overexpression of high mobility group A1 protein in human uveal melanomas: - Overexpression of high mobility group A1 protein in human uveal melanomas:

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HMGA1 induces intestinal polyposis in transgenic mice and drives tumor - HMGA1 induces intestinal polyposis in transgenic mice and drives tumor

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Correlation of overexpression of HMGA1 and HMGA2 with poor tumor differentiation, - Correlation of overexpression of HMGA1 and HMGA2 with poor tumor differentiation,

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HMGA2 maintains oncogenic RAS-induced epithelial-mesenchymal transition in human - HMGA2 maintains oncogenic RAS-induced epithelial-mesenchymal transition in human

Watanabe S, Ueda Y, Akaboshi S, Hino Y, Sekita Y, Nakao M. Am J Pathol. 2009 Mar;174(3):854-68.

Increased expression of high mobility group A proteins in lung cancer. - Increased expression of high mobility group A proteins in lung cancer.

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