Anti-HMGA2 antibody
| Name | Anti-HMGA2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab97276 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ChIP WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474) |
| Description | Rabbit Polyclonal |
| Gene | HMGA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilutionLane 1 : HepG2Lane 2 : HCT116Lysates/proteins at 30 µg per lane.