Anti-HMGCR antibody [EPR1685(N)]

• Supplier: Abcam
• Catalog: ab174830
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1685(N)
• Applications: IHC-P WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HMGCR aa 400-500
• Description: Rabbit Monoclonal
• Gene: HMGCR
• Conjugate: Unconjugated

Product images

All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/300 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : TT cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/3000 dilution (purified)Lane 1 : Jurkat cell lysateLane 2 : TT cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/300 dilution (unpurified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/3000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/1000 dilution (unpurified)Lane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : TT cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCR with unpurified ab174830 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCR with purified ab174830 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HMGCR with unpurified ab174830 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling HMGCR with unpurified ab174830 at 1/100 dilution.

ab174830 (unpurified) at 1/10 immunoprecipitating HMGCR in HepG2 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab174830 (purified) at 1/100 immunoprecipitating HMGCR in HepG2 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Product References

Deletion of PARP-2 induces hepatic cholesterol accumulation and decrease in HDL - Deletion of PARP-2 induces hepatic cholesterol accumulation and decrease in HDL

Szanto M, Brunyanszki A, Marton J, Vamosi G, Nagy L, Fodor T, Kiss B, Virag L, Gergely P, Bai P. Biochim Biophys Acta. 2014 Apr;1842(4):594-602. doi:

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is - A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is

Hu YW, Yang JY, Ma X, Chen ZP, Hu YR, Zhao JY, Li SF, Qiu YR, Lu JB, Wang YC, Gao JJ, Sha YH, Zheng L, Wang Q. J Lipid Res. 2014 Apr;55(4):681-97.