Anti-HNF-4-alpha + HNF-4-gamma antibody
| Name | Anti-HNF-4-alpha + HNF-4-gamma antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64911 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide corresponding to Human HNF-4-alpha + HNF-4-gamma (internal sequence) |
| Description | Rabbit Polyclonal |
| Gene | HNF4A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911) at 1/500 dilutionLane 1 : extracts from HeLa cellsLane 2 : extracts from HepG2 cellsLane 3 : extracts from 293 cellsLane 4 : extracts from 293 cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
ICC/IF image of ab64911 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64911, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.