Anti-hnRNP C1 + C2 antibody [4F4]
| Name | Anti-hnRNP C1 + C2 antibody [4F4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10294 |
| Prices | $397.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4F4 |
| Applications | ICC/IF ICC/IF IP IHC-P ELISA WB |
| Species Reactivities | Mouse, Chicken, Hamster, Human, Monkey |
| Antigen | RNP’s eluted from oligo (dT) cellulose column |
| Description | Mouse Monoclonal |
| Gene | HNRNPC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-hnRNP C1 + C2 antibody [4F4] (ab10294) at 1/1000 dilutionLane 1 : Nuclear lysate, HEK293A cellsLane 2 : Cytoplasmic lysate, HEK293A cellsLysates/proteins at 30 µg per lane.SecondaryHRP conjugated ImmunoPure Goat Anti-Mouse IgG (H+L) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3300_ab10294_1.jpg)
All lanes : Anti-hnRNP C1 + C2 antibody [4F4] (ab10294) at 1/1000 dilutionLane 1 : Nuclear lysate, HEK293A cellsLane 2 : Cytoplasmic lysate, HEK293A cellsLysates/proteins at 30 µg per lane.SecondaryHRP conjugated ImmunoPure Goat Anti-Mouse IgG (H+L) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab10294 (1µg/ml) staining hnRNP in human placenta, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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