ab128049 at 1/100 staining hnRNP C1+C2 in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.
2 µg of ab128049 was added to aliquots of cleared HeLa cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded. Lane 1: Cell Lysate control Lane 2: Cell lysate with ab128049 Lane 3: Cell lysate without ab128049 Lane 4: V5 tag control
ChIP was successfully performed using ab128049. Lane 1: Input Lane 2: IgG Lane 3: First elution Lane 4: Second elution
Quantitative analysis of ChIPd DNA species. Left: Distribution and abundance of ChIPd DNAs. Right: Computer-regenerated image of negative control and anti-HNRPC-ChIPd DNA.