Anti-hnRNP D/AUF1 antibody
| Name | Anti-hnRNP D/AUF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab50692 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Human, Mouse, Rat, Bovine, Dog, Zebrafish |
| Antigen | A region within synthetic peptide: AAAAAGSGAG TGGGTASGGT EGGSAESEGA KIDASKNEED EGKMFIGGLS, corresponding to N terminal amino acids 37-86 of Human hnRNP D/AUF1 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | HNRNPD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-hnRNP D/AUF1 antibody (ab50692) at 1.25 µg/mlLane 1 : MW ladderLane 2 : Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Staining of hnRNP D/AUF1 on human intestine tissue sections, using ab50692 at 8ug/ml. Expression is detected on the epithelial cells of the intestinal villus (arrows).
ab50692 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50692 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Downregulation of Nipah virus N mRNA occurs through interaction between its 3' - Downregulation of Nipah virus N mRNA occurs through interaction between its 3'
Hino K, Sato H, Sugai A, Kato M, Yoneda M, Kai C. J Virol. 2013 Jun;87(12):6582-8.
Regulation of thrombospondin-1 expression through AU-rich elements in the 3'UTR - Regulation of thrombospondin-1 expression through AU-rich elements in the 3'UTR
McGray AJ, Gingerich T, Petrik JJ, Lamarre J. Cell Mol Biol Lett. 2011 Mar;16(1):55-68.
A method for purification, identification and validation of DNMT1 mRNA binding - A method for purification, identification and validation of DNMT1 mRNA binding
Unterberger A, Torrisani J, Szyf M. Biol Proced Online. 2008;10:47-57.