Anti-hnRNP K antibody
| Name | Anti-hnRNP K antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab32969 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Chicken, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human hnRNP K |
| Description | Rabbit Polyclonal |
| Gene | HNRNPK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of hnRNP K staining in Human Colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32969, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![hnRNP K was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP K and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32969.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 50kDa; hnRNP K](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3365_ab32969-171829-IPV018ab329693min.jpg)
hnRNP K was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP K and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32969.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 50kDa; hnRNP K
All lanes : Anti-hnRNP K antibody (ab32969) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
All lanes : Anti-hnRNP K antibody (ab32969) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : Brain (Mouse) Tissue LysateLane 4 : Testis (Mouse) Tissue Lysate - normal tissueLane 5 : Ovary (Mouse) Tissue Lysate - normal tissueLane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 7 : Brain (Rat) Tissue Lysate - normal tissueLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab32969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
The RNA binding protein hnRNP-K mediates post-transcriptional regulation of - The RNA binding protein hnRNP-K mediates post-transcriptional regulation of
Tahir TA, Singh H, Brindle NP. Cell Signal. 2014 Jul;26(7):1379-84.
Hnrnpk, a protein differentially expressed in immature rat ovarian development, - Hnrnpk, a protein differentially expressed in immature rat ovarian development,
Wang N, Zhang P, Guo X, Zhou Z, Sha J. Endocrinology. 2011 Mar;152(3):1024-35.
Comparative proteome profile of immature rat ovary during primordial follicle - Comparative proteome profile of immature rat ovary during primordial follicle
Wang N, Zhang P, Guo X, Xie J, Huo R, Wang F, Chen L, Shen J, Zhou Z, Shi Q, Zhao B, Sha J. Proteomics. 2009 Jul;9(13):3425-34.