Anti-hnRNP K antibody
| Name | Anti-hnRNP K antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18195 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Chicken, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human hnRNP K |
| Blocking Peptide | Human hnRNP K peptide |
| Description | Rabbit Polyclonal |
| Gene | HNRNPK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of hnRNP K staining in Human Colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18195, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab18195 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18195, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). Panel A (left) shows localisation of the antibody (green) to specific foci in the nucleus, panel B (right) does not show the Alexa Fluor® 488 channel for comparison.
All lanes : Anti-hnRNP K antibody (ab18195) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line)Lane 3 : Testis (Mouse) Tissue LysateLane 4 : Ovary (Mouse) Tissue LysateLane 5 : PC12 (Rat adrenal pheochromocytoma cell line)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
ab18195 staining hnRNP K in HeLa cells treated with PD 98059 (ab120234), by ICC/IF. Changes in localization of hnRNP K (translocation from cytoplasm to nucleous) correlates with increased concentration of hnRNP K, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of ab120234 ( hnRNP K) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18195(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Product References
Kinase Inhibitor Screening Identifies Cyclin-Dependent Kinases and Glycogen - Kinase Inhibitor Screening Identifies Cyclin-Dependent Kinases and Glycogen
Moujalled D, James JL, Parker SJ, Lidgerwood GE, Duncan C, Meyerowitz J, Nonaka T, Hasegawa M, Kanninen KM, Grubman A, Liddell JR, Crouch PJ, White AR. PLoS One. 2013 Jun 26;8(6):e67433. Print 2013.
Granzyme M targets host cell hnRNP K that is essential for human cytomegalovirus - Granzyme M targets host cell hnRNP K that is essential for human cytomegalovirus
van Domselaar R, de Poot SA, Remmerswaal EB, Lai KW, ten Berge IJ, Bovenschen N. Cell Death Differ. 2013 Mar;20(3):419-29.
Granzyme K displays highly restricted substrate specificity that only partially - Granzyme K displays highly restricted substrate specificity that only partially
Bovenschen N, Quadir R, van den Berg AL, Brenkman AB, Vandenberghe I, Devreese B, Joore J, Kummer JA. J Biol Chem. 2009 Feb 6;284(6):3504-12.
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Rahman-Roblick R, Roblick UJ, Hellman U, Conrotto P, Liu T, Becker S, Hirschberg D, Jornvall H, Auer G, Wiman KG. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5401-6. Epub 2007 Mar 19.