Anti-hnRNP K antibody [F45 P9 C7]
| Name | Anti-hnRNP K antibody [F45 P9 C7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab23644 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | F45 P9 C7 |
| Applications | ICC/IF ICC/IF FC WB IHC-P |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Chicken |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human hnRNP K |
| Description | Mouse Monoclonal |
| Gene | HNRNPK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Lane 1 : MarkerLanes 2 - 5 : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 1 µg/mlLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µgLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µgLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 20 µgSecondaryLanes 2 - 5 : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3395_ab23644%2520AP237728.jpg)
Lane 1 : MarkerLanes 2 - 5 : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 1 µg/mlLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µgLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µgLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 20 µgSecondaryLanes 2 - 5 : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilutionPerformed under reducing conditions.
Formalin-fixed, paraffin embedded tissue sections from human normal colon or human colon carcinoma stained with ab23644 mouse monoclonal to hnRNP K. hnRNP K expression is increased in the tumour tissue.
ICC/IF image of ab23644 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23644, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.
ab23644 staining hnRNP K in Human head and neck tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation with Tris EDTA (pH9). Samples were incubated with primary antibody (1 µg/ml in TBST) for 16 hours at 4°C. An undiluted HRP-conjugated Rabbit anti-mouse polyclonal was used as the secondary antibody.See Abreview
![All lanes : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 5 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Testis (Mouse) Tissue Lysate Lane 4 : Lung (Mouse) Tissue LysateLane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3399_hnRNP-K-Primary-antibodies-ab23644-6.jpg)
All lanes : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 5 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Testis (Mouse) Tissue Lysate Lane 4 : Lung (Mouse) Tissue LysateLane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab23644 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23644, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3400_hnRNP-K-Primary-antibodies-ab23644-7.jpg)
Overlay histogram showing HeLa cells stained with ab23644 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23644, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunofluorescence analysis of SH-SY5Y cells staining hnRNP K with ab23644. Cells were fixed with paraformaldehyde in PBS for 30 min and permeabilized with 90% chilled methanol for 5 min. After blocking for 1 hour with 10% normal goat serum, cells were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®-conjugated goat anti-mouse IgG (1/500) was used as the secondary antibody.
Product References
All human granzymes target hnRNP K that is essential for tumor cell viability. - All human granzymes target hnRNP K that is essential for tumor cell viability.
van Domselaar R, Quadir R, van der Made AM, Broekhuizen R, Bovenschen N. J Biol Chem. 2012 Jun 29;287(27):22854-64.
C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced - C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced
Meyerowitz J, Parker SJ, Vella LJ, Ng DCh, Price KA, Liddell JR, Caragounis A, Li QX, Masters CL, Nonaka T, Hasegawa M, Bogoyevitch MA, Kanninen KM, Crouch PJ, White AR. Mol Neurodegener. 2011 Aug 8;6:57.
Heterogeneous ribonucleoprotein K is a marker of oral leukoplakia and correlates - Heterogeneous ribonucleoprotein K is a marker of oral leukoplakia and correlates
Matta A, Tripathi SC, DeSouza LV, Grigull J, Kaur J, Chauhan SS, Srivastava A, Thakar A, Shukla NK, Duggal R, DattaGupta S, Ralhan R, Michael Siu KW. Int J Cancer. 2009 Sep 15;125(6):1398-406.