All lanes : Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilutionLane 1 : K562 cell lysate with NFDM/TBSTLane 2 : Jurkat cell lysate with NFDM/TBSTLane 3 : 293 cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Immunofluorescence analysis of -20℃ acetone-fixed Jurkat cells labeling hnRNP U using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.