Anti-Hormone sensitive lipase antibody
| Name | Anti-Hormone sensitive lipase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45422 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic 14 amino acid peptide from near the carboxy terminus of human HSL/LIPE |
| Blocking Peptide | Human Hormone sensitive lipase peptide |
| Description | Rabbit Polyclonal |
| Gene | LIPE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab45422 staining Hormone sensitive lipase in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.See Abreview
Lane 1 : Anti-Hormone sensitive lipase antibody (ab45422) at 0.5 µg/mlLane 2 : Anti-Hormone sensitive lipase antibody (ab45422) at 1 µg/mlLane 1 : Human lymph node tissue lysateLane 2 : Human lymph node tissue lysateLysates/proteins at 15 µg per lane.
Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.
Product References
Angiotensin II type 2 receptor promotes adipocyte differentiation and restores - Angiotensin II type 2 receptor promotes adipocyte differentiation and restores
Shum M, Pinard S, Guimond MO, Labbe SM, Roberge C, Baillargeon JP, Langlois MF, Alterman M, Wallinder C, Hallberg A, Carpentier AC, Gallo-Payet N. Am J Physiol Endocrinol Metab. 2013 Jan 15;304(2):E197-210. doi:
Suppression of adipose lipolysis by long-chain fatty acid analogs. - Suppression of adipose lipolysis by long-chain fatty acid analogs.
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Skeletal muscle growth hormone receptor signaling regulates basal, but not - Skeletal muscle growth hormone receptor signaling regulates basal, but not
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Central endocannabinoid signaling regulates hepatic glucose production and - Central endocannabinoid signaling regulates hepatic glucose production and
O'Hare JD, Zielinski E, Cheng B, Scherer T, Buettner C. Diabetes. 2011 Apr;60(4):1055-62.
Short-term regulation of tumor necrosis factor-alpha-induced lipolysis in 3T3-L1 - Short-term regulation of tumor necrosis factor-alpha-induced lipolysis in 3T3-L1
Lien CC, Au LC, Tsai YL, Ho LT, Juan CC. Endocrinology. 2009 Nov;150(11):4892-900.
Hepatic overexpression of hormone-sensitive lipase and adipose triglyceride - Hepatic overexpression of hormone-sensitive lipase and adipose triglyceride
Reid BN, Ables GP, Otlivanchik OA, Schoiswohl G, Zechner R, Blaner WS, Goldberg IJ, Schwabe RF, Chua SC Jr, Huang LS. J Biol Chem. 2008 May 9;283(19):13087-99.