LIPE

Gene Symbol LIPE
Entrez Gene 3991
Alt Symbol AOMS4, FPLD6, HSL, LHS
Species Human
Gene Type protein-coding
Description lipase, hormone-sensitive
Other Description hormone-sensitive lipase|hormone-sensitive lipase testicular isoform
Swissprots Q6NSL7 Q3LRT2 Q05469
Accessions AAA69810 ABA03168 EAW57135 Q05469 AK292482 BAF85171 AK309893 BC029301 BC029961 BC070041 AAH70041 U40002 AAC50666 XM_005258937 XP_005258994 XM_005258938 XP_005258995 XM_005258939 XP_005258996 XM_005258940 XP_005258997 XM_005258941 XP_005258998 XM_006723218 XP_006723281 NM_005357 NP_005348
Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Subcellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Note=Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
Top Pathways Insulin signaling pathway, AMPK signaling pathway, cAMP signaling pathway

Phospho-HSL (Ser660) Antibody - 4126 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from differentiated NIH/3T3-L1 cells treated with isoproterenol, using Phospho-HSL (Ser660) Antibody.

Phospho-HSL (Ser565) Antibody - 4137 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from differentiated 3T3-L1 cells treated with forskolin, oligomycin or lambda protein phosphatase, using Phospho-HSL (Ser565) Antibody.

Phospho-HSL (Ser563) Antibody - 4139 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from differentiated 3T3-L1 cells treated with isoproterenol or lambda protein phosphatase, using Phospho-HSL (Ser563) Antibody.

HSL Antibody - 4107 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using HSL Antibody.

HSL (G-7) - sc-74489 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSL (G-7): sc-74489. Western blot analysis of HSL expression in BT-20 (A) and SK-BR-3 (B) whole cell lysates.

HSL (H-300) - sc-25843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HSL (H-300): sc-25843. Western blot analysis of HSL expression in BT-20 whole cell lysate.

HSL (N-19) - sc-27953 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HSL (P-20) - sc-27954 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Hormone sensitive lipase (phospho S552) antibody - ab195945 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hormone sensitive lipase (phospho S552) antibody (ab195945) at 1/500 dilutionLane 1 : K562 whole cell lysates with antigen-specific peptideLane 2 : K562 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] - ab109400 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2329(2)
All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilutionLane 1 : Human skeletal muscle lysatesLane 2 : Human skeletal muscle lysates treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.

Anti-Hormone sensitive lipase (phospho S855) antibody - ab55228 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228) at 1/500 dilutionLane 1 : extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours).Lane 2 : extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours), and treated with the immunising phosphopeptide.Lysates/proteins at 5 µg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)

Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] - ab76492 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2330
All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilutionLane 1 : Human skeletal muscle lysate, untreatedLane 2 : Human skeletal muscle lysate, treated with LPLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/1000 dilution

Anti-Hormone sensitive lipase antibody - ab45422 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab45422 staining Hormone sensitive lipase in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.See Abreview

Anti-Hormone sensitive lipase antibody - ab17652 from Abcam

Host Chicken
Clonality Polyclonal
All lanes : Anti-Hormone sensitive lipase antibody (ab17652) at 1/1000 dilutionLane 1 : Whole tissue lysate from mouse white adipose tissueLane 2 : Whole tissue lysate from mouse white adipose tissueLysates/proteins at 50 µg per lane.SecondaryHRP conjugated goat anti-chicken antibodydeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 82 kDa (why is the actual band size different from the predicted?)Additional bands at : 140 kDa (possible non-specific binding).Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-Hormone sensitive lipase antibody - ab63492 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hormone sensitive lipase antibody (ab63492) at 1/500 dilutionLane 1 : extracts from Jurkat cells, treated with Calyculin A (100nM, 30mins)Lane 2 : extracts from Jurkat cells, treated with Calyculin A (100nM, 30mins) with blocking peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-Hormone sensitive lipase antibody - ab73736 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hormone sensitive lipase antibody (ab73736) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from MCF-7 cellsLane 3 : Extracts from Jurkat cells plus 10µg immunizing peptideLysates/proteins at 30 µg per lane.

Anti-Hormone sensitive lipase antibody - ab28958 from Abcam

Prices $370.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal

Anti-Hormone sensitive lipase antibody - ab103281 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Hormone sensitive lipase antibody (ab103281) at 1/50 dilution + HL-60 cell lysate at 35 µg

Anti-LIPE / HSL Antibody (Pro548) - LS-C175887 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of HSL pAb in paraffin-embedded human breast carcinoma tissue.

Anti-LIPE / HSL Antibody (phospho-Ser855/Ser554) - LS-C199085 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-LIPE / HSL Antibody (phospho-Ser552) - LS-C199084 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma, using HSL (Phospho-Ser552) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-LIPE / HSL Antibody (aa302-425, clone 729923) - LS-C149969 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 729923
Hormone-sensitive Lipase/HSL in Human Testis. Hormone-sensitive Lipase/HSL was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody at 8 ug/ml overnight at 4°C. Tissue was stained using Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of spermatocytes.

Anti-LIPE / HSL Antibody (aa518-567) - LS-C117614 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human tonsil tissue, using HSL (Ab-552) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-LIPE / HSL Antibody (aa822-849) - LS-C165793 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human testis tissue reacted with LIPE Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-LIPE / HSL Antibody (Ala546) - LS-C177164 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of HSL (A546) pAb in paraffin-embedded human tonsil tissue.
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