Anti-HPRT antibody
| Name | Anti-HPRT antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10479 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Chicken, Rat, Hamster |
| Antigen | Synthetic peptide derived from residues 200 to the C-terminus of Human HPRT |
| Blocking Peptide | Human HPRT peptide |
| Description | Rabbit Polyclonal |
| Gene | HPRT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab10479 stained human HeLa cells. The cells were methanol fixed ( 5 min) and incubated with the antibody (ab10479, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
All lanes : Anti-HPRT antibody (ab10479) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilutionPerformed under reducing conditions.
All lanes : Anti-HPRT antibody (ab10479) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlas. ab10479 staining HPRT in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab10479 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab10479 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Anti-HPRT antibody (ab10479) at 1/500 dilution + Human HPRT full length protein (ab97411) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Product References
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