Anti-HS3ST1 antibody
| Name | Anti-HS3ST1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab91065 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Hamster, Mouse, Rat, Rabbit, Horse, Guinea Pig, Bovine, Dog |
| Antigen | Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (KGFYCLRDSG RDRCLHESKG RAHPQVDPKL LNKLHEYFHE PNKKFFELVG) of Human HS3ST1 (NP_005105) |
| Description | Rabbit Polyclonal |
| Gene | HS3ST1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-HS3ST1 antibody (ab91065) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature and permeabilized with permeabilization buffer + 10% FBS in PBS for 10 minutes. Cells were incubated with ab91065 and goat anti-GM130 Golgi marker (ab1299) overnight at 4°C in the dark. Cells were washed once and stained with Alexa Fluor 647-conjugated donkey anti-rabbit IgG and Alexa Fluor 488-conjugated donkey anti-goat IgG secondary antibodies. DAPI - DNA. Dual-29 cells were used as a positive control, and the other cell samples were then evaluated at the same frequency. Overexpression of HS3st1 in Dual-29 cells resulted in a broad distribution of HS3st1. Overexpression of HS3st1 in Dual-10 cells localized HS3st1 in the Golgi. Overexpression of Golgi-targeted HS3st1 in CHO-gt31 localized HS3st1 in the Golgi.