HS3ST1

Gene Symbol	HS3ST1
Entrez Gene	9957
Alt Symbol	3OST, 3OST1
Species	Human
Gene Type	protein-coding
Description	heparan sulfate (glucosamine) 3-O-sulfotransferase 1
Other Description	3-OST-1\|h3-OST-1\|heparan sulfate 3-O-sulfotransferase 1\|heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1\|heparan sulfate glucosamine 3-O-sulfotransferase 1\|heparin-glucosamine 3-O-sulfotransferase
Swissprots	B3KUA6 O14792 Q6PEY8
Accessions	EAW92699 EAW92700 EAW92701 EAW92702 O14792 AA825270 AF019386 AAB84388 AI478981 AK096823 BAG53368 BC025735 AAH25735 BC057803 AAH57803 BM547276 HQ448559 ADQ33038 XM_011513913 XP_011512215 NM_005114 NP_005105
Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. {ECO:0000269\|PubMed:9346953}.
Subcellular Location	Golgi apparatus lumen {ECO:0000305}.
Tissue Specificity	Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta. {ECO:0000269\|PubMed:9988767}.
Top Pathways	Glycosaminoglycan biosynthesis - heparan sulfate / heparin

HS3ST1 (C-15) - sc-104313 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HS3ST1 (C-15): sc-104313. Western blot analysis of HS3ST1 expression in mouse kidney tissue extract.

HS3ST1 (D-16) - sc-104314 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HS3ST1 (D-16): sc-104314. Western blot analysis of HS3ST1 expression in U-87 MG whole cell lysate.

HS3ST1 (L-13) - sc-104315 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-HS3ST1 antibody - ab91065 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HS3ST1 antibody (ab91065) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-HS3ST1 antibody - ab70080 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-HS3ST1 antibody (ab70080) at 1/500 dilutionLane 1 : HS3ST1 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-HS3ST1 antibody - ab190286 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

Anti-HS3ST1 antibody (ab190286) at 1 µg + Human cerebellum lysate at 35 µgdeveloped using the ECL technique

Anti-HS3ST1 Antibody - LS-C308315 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-HS3ST1 Antibody - LS-C310662 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HS3ST1 Antibody (C-Terminus) - LS-C109741 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

HS3ST1 antibody LS-C109741 Western blot of Fetal Stomach lysate.

Anti-HS3ST1 Antibody (aa283-295) - LS-C305872 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

HS3ST1 antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody - AF5968 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human HS3ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HS3ST1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody - NBP1-89375 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375] - Analysis in human brain tissue.

Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody - NBP1-58058 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-58058] - Titration: 0.2-1 ug/ml, Positive Control: Human Stomach.

Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody - H00009957-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody - H00009957-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HS3ST1 antibody produced in mouse - SAB1406902 from Sigma-Aldrich

Prices	$347.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-HS3ST1: <B>Cat. No. SAB1406902</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~33.8 kDa using HS3ST1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-HS3ST1 antibody produced in rabbit - HPA002237 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-HS3ST1: <B>Cat. No. HPA002237</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cerebral cortex tissue showing positive staining of neuronal and non-neuronal cells.

Anti-HS3ST1 antibody produced in rabbit - SAB1411710 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-HS3ST1: <B>Cat. No. SAB1411710</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~35.8 kDa using HS3ST1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-HS3ST1 antibody produced in rabbit - SAB2104135 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-HS3ST1, antibody produced in rabbit: <B>Cat. No. SAB2104135</B>: Immunoblotting analysis of HS3ST1 in fetal stomach tissue lysate with HS3ST1 anitbody at 1 μg/mL.

HS3ST1 antibody - middle region (ARP33092_P050) - ARP33092_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

HS3ST1-Antibody-C-Term - AF4106a from Abgent, a WuXi AppTec company

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AF4106a (1 µg/ml) staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody - AF5968 from R&D Systems

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep

Detection of Human HS3ST1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human HS3ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for HS3ST1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

Human HS3ST1 Affinity Purified Polyclonal Ab, Sheep IgG - AF5968-SP from R&D Systems

Host	Sheep
Clonality	Polyclonal

HS3ST1 antibody - 70R-17817 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti -HS3ST1 (Heparan Sulfate Glucosamine 3-O-Sulfotransferase 1, 3OST, 3OST1) - 211349 from USBiological Life Science

Host	Rabbit
Clonality	Polyclonal

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