Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane, cytoplasm and centrosome. Recommended concentration of ab121569 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121569 at 1/450 dilution staining HSCB in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
All lanes : Anti-HSCB antibody (ab121569) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : HSCB transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))developed using the ECL technique