HSCB

Gene Symbol	HSCB
Entrez Gene	150274
Alt Symbol	DNAJC20, HSC20, JAC1, dJ366L4.2
Species	Human
Gene Type	protein-coding
Description	HscB mitochondrial iron-sulfur cluster co-chaperone
Other Description	DnaJ (Hsp40) homolog, subfamily C, member 20\|HscB iron-sulfur cluster co-chaperone homolog\|J-type co-chaperone HSC20\|dnaJ homolog subfamily C member 20\|iron-sulfur cluster co-chaperone protein HscB, mitochondrial
Swissprots	Q8IWL3 Q9BWS7
Accessions	EAW59758 EAW59759 EAW59760 Q8IWL3 AY191719 AAN85282 BC065569 AAH65569 CR456462 CAG30348 XM_005261359 XP_005261416 XM_005261360 XP_005261417 XM_005261361 XP_005261418 XR_937815 NM_172002 NP_741999
Function	Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria. {ECO:0000269\|PubMed:20668094}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:20668094}. Mitochondrion {ECO:0000269\|PubMed:20668094}.
Tissue Specificity	Expressed in lung, brain, stomach, spleen, ovary, testis, liver, muscle and heart. {ECO:0000269\|PubMed:12938016, ECO:0000269\|PubMed:20668094}.

HscB (C-19) - sc-79525 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HscB (P-13) - sc-79528 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-HSCB antibody - ab121569 from Abcam

Prices	$443.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane, cytoplasm and centrosome. Recommended concentration of ab121569 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Anti-HSCB Antibody (clone 2D10) - LS-C338452 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2D10

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSCB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSCB.

Anti-HSCB Antibody (clone 2A7) - LS-C340624 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2A7

Anti-HSCB Antibody (clone 2B7) - LS-C338453 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2B7

Anti-HSCB Antibody (clone 3E1) - LS-C338448 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3E1

IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

HSCB Antibody - NBP1-84066 from Novus Biologicals

Prices	$129.00, $419.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: HSCB Antibody [NBP1-84066] - Analysis in control (vector only transfected HEK293T lysate) and hSCB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

HSCB Antibody - NBP2-16883 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: HSCB Antibody [NBP2-16883] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.

HSCB Antibody - H00150274-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: HSCB Antibody [H00150274-B01P] - Analysis of HSCB expression in transfected 293T cell line by HSCB polyclonal antibody. Lane 1: RP3-366L4.2 transfected lysate(25.85 KDa). Lane 2: Non-transfected lysate.

Anti-HSCB antibody produced in rabbit - HPA018447 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-HSCB: <B>Cat. No. HPA018447</B>: Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-HSCB antibody produced in rabbit - SAB2701280 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-HSCB: <B>Cat. No. SAB2701280</B>: Western Blot analysis of HSCB in extracts from <B>Lanes</B><BR/>A. 293T using <B>Cat. No. SAB2701280</B> at a 1:1000 dilution.

Anti-HSCB antibody produced in mouse - SAB1408915 from Sigma-Aldrich

Prices	$347.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-HSCB: <B>Cat. No. SAB1408915</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~25.9 kDa using HSCB transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

HscB antibody [N1C3] - GTX116372 from GeneTex

Prices	$119.00
Sizes	25 μl

Anti-HscB antibody [N1C3] used in Western blot. GTX116372

HSCB / DNAJC20 / HSC20 antibody - 15132-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15132-1-AP( HSCB Antibody) at dilution of 1:50 (under 40x lens)

HSCB / DNAJC20 / HSC20 antibody - H00150274-B01P from Acris Antibodies

Prices	$360.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

HSCB antibody - 19846-1-AP from Proteintech Group

Host	Rabbit
Clonality	Polyclonal

HSCB antibody - 15132-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 47 μg/150μl
Host	Rabbit
Clonality	Polyclonal

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15132-1-AP( HSCB Antibody) at dilution of 1:1000

HSCB, Polyclonal Antibody - MBS2516485 from MyBioSource

Prices	$195.00, $270.00, $435.00
Sizes	60 µl, 120 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

HSCB, Polyclonal Antibody - MBS2524463 from MyBioSource

Prices	$195.00, $270.00, $435.00
Sizes	60 µl, 120 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

HscB - MBS272761 from MyBioSource

Prices	$360.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000

HSCB Antibody - EPP14096 from Elabscience

Prices	$296.00, $485.00
Sizes	120 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

HSCB Antibody - EPP14097 from Elabscience

Prices	$296.00, $485.00
Sizes	120 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

Anti-HSCB (aa 160-232) polyclonal antibody - DPABH-18852 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

Anti-HSCB monoclonal antibody - DCABH-200707 from Creative Diagnostics

Host	Rabbit
Clonality	Monoclonal

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