Anti-HSD11B1 antibody
| Name | Anti-HSD11B1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83522 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Pig, Mouse, Rat, Sheep, Rabbit, Horse, Guinea Pig, Bovine, Cat, Dog |
| Antigen | Synthetic peptide derived from within N terminal amino acids 72-121 (QKVVSHCLEL GAASAHYIAG TMEDMTFAEQ FVAQAGKLMG GLDMLILNHI), of Human HSD11B1 (NP_005516) Run BLAST with Run BLAST with |
| Blocking Peptide | Human HSD11B1 peptide |
| Description | Rabbit Polyclonal |
| Gene | Hsd11b1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-HSD11B1 antibody (ab83522) at 1 µg/ml (in 5% skim milk/ PBS) + Human fetal liver lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
All lanes : Anti-HSD11B1 antibody (ab83522) at 1/2000 dilution (in PBS + 0.1% Tween + 2.5% milk for 90 minutes at 22°C)Lane 1 : Tissue lysate of Human liver microsomesLane 2 : Tissue lysate of Mouse liver microsomesLane 3 : Tissue lysate of Pig liver microsomesLysates/proteins at 25 µg per lane.SecondaryAn HRP-conjugated Donkey anti-rabbit polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab83522 at 5 µg/ml staining HSD11B1 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab83522 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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