All lanes : Anti-HSD17B2 antibody [3E9] (ab127992) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA for 48 hrs and lysedLane 2 : HEK293T cells transfected with pCMV6-ENTRY HSD17B2 cDNA for 48 hrs and lysedLysates/proteins at 5 µg per lane.
ab127992, at a 1/100 dilution, staining HSD17B2 in HEK293T cells transfected with either HSD17B2 overexpressing plasmid (red) or empty vector control plasmid (blue), by Flow Cytometry.
ab127992, at a 1/100 dilution, staining HSD17B2 in Jurkat cells (red) by Flow Cytometry, compared to a nonspecific negative control antibody (blue).