Anti-HSD3B1 antibody
| Name | Anti-HSD3B1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55268 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | FC WB IHC-P IHC-F |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-374 of Human HSD3B1 |
| Description | Mouse Monoclonal |
| Gene | HSD3B1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
HSD3B1 antibody (ab55268) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.
Anti-HSD3B1 antibody (ab55268) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
 used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/4510_HSD3B1-Primary-antibodies-ab55268-6.jpg)
Overlay histogram showing JEG3 cells stained with ab55268 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55268, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Neurosteroid-mediated regulation of brain innate immunity in HIV/AIDS: DHEA-S - Neurosteroid-mediated regulation of brain innate immunity in HIV/AIDS: DHEA-S
Maingat FG, Polyak MJ, Paul AM, Vivithanaporn P, Noorbakhsh F, Ahboucha S, Baker GB, Pearson K, Power C. FASEB J. 2013 Feb;27(2):725-37.
Steroid synthesis by primary human keratinocytes; implications for skin disease. - Steroid synthesis by primary human keratinocytes; implications for skin disease.
Hannen RF, Michael AE, Jaulim A, Bhogal R, Burrin JM, Philpott MP. Biochem Biophys Res Commun. 2011 Jan 7;404(1):62-7. doi: