HSD3B1

Gene Symbol HSD3B1
Entrez Gene 3283
Alt Symbol 3BETAHSD, HSD3B, HSDB3, HSDB3A, I, SDR11E1
Species Human
Gene Type protein-coding
Description hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Other Description 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1|3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I|3-beta-HSD I|3-beta-hydroxy-5-ene steroid dehydrogenase|3-beta-hydroxy-Delta(5)-steroid dehydrogenase|delta-5-3-ketosteroid isomerase|hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1|progesterone reductase|short chain dehydrogenase/reductase family 11E, member 1|steroid Delta-isomerase|trophoblast antigen FDO161G
Swissprots P14060 A8K691 Q8IV65 Q14545
Accessions AAA36001 AAA51662 AAA51831 CBH19320 EAW56703 EAW56704 P14060 AK291556 BAF84245 AL544564 BC031999 AAH31999 DQ891161 ABM82087 DQ894342 ABM85268 M27137 AAA36015 M35493 AAA51538 S45679 AAB23543 X53321 CAA37408 X55997 CAA39469 XM_011541314 XP_011539616 NM_000862 NP_000853
Function 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. {ECO:0000269|PubMed:1401999, ECO:0000269|PubMed:2139411}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue Specificity Placenta and skin. Predominantly expressed in mammary gland tissue.
Top Pathways Steroid hormone biosynthesis, Ovarian steroidogenesis

3β-HSD (P-18) - sc-30820 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
3β-HSD (P-18): sc-30820. Western blot analysis of 3β-HSD expression in mouse testis (A) and rat adrenal gland (B) tissue extracts.

3β-HSD (37-2) - sc-100466 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
3β-HSD (37-2): sc-100466. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing membrane and cytoplasmic localization.

Anti-HSD3B1 antibody - ab55268 from Abcam

Prices $380.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
HSD3B1 antibody (ab55268) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.

Anti-HSD3B1 antibody - ab80363 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-HSD3B1 antibody (ab80363) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-HSD3B1 antibody - ab48018 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-HSD3B1 antibody (ab48018) at 0.01 µg/ml + Human Placenta lysate at 35 µgdeveloped using the ECL technique

Anti-HSD3B1 antibody [EPR9686] - ab150384 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9686
Overlay histogram showing JEG3 cells stained with ab150384 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150384, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HSD3B1 antibody [EPR9687] - ab167417 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9687
Anti-HSD3B1 antibody [EPR9687] (ab167417) at 1/1000 dilution + Human placenta cell lysate at 10 µgSecondaryGoat anti-rabbit HRP antibody at 1/2000 dilution

Anti-HSD3B1 antibody [FDO66Q] - ab69807 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone FDO66Q
ab69807, at 1:1000 dilution, staining human HSD3B1 in placental villi by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-HSD3B2 antibody - ab154385 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSD3B2 antibody (ab154385) at 1/1000 dilutionLane 1 : Raji whole cell lysateLane 2 : HeLaS3 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-HSD3B2 antibody - ab80500 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-HSD3B2 antibody (ab80500) at 1 µg/ml + 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-HSD3B2 antibody - ab191515 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSD3B2 antibody (ab191515)Lane 1 : HEK293T whole cell lysateLane 2 : Raw264.7 whole cell lysateLane 3 : H9C2 whole cell lysate

Anti-HSD3B2 antibody - ab154159 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HSD3B2 antibody (ab154159) at 1/500 dilutionLane 1 : HeLa S3 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-HSD3B2 antibody [373CT9.1.3] - ab75710 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 373CT9.1.3
Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HSD3B1 Antibody (clone FDO66Q, DY488) - LS-C141807 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone FDO66Q

Anti-HSD3B1 Antibody (clone FDO66Q, Biotin) - LS-C141805 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone FDO66Q

Anti-HSD3B1 Antibody (clone FDO66Q, DY650) - LS-C141806 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone FDO66Q

Anti-HSD3B1 Antibody (clone FDO66Q, HRP) - LS-C141808 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone FDO66Q

Anti-HSD3B1 Antibody (clone FDO66Q, DY550) - LS-C141809 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone FDO66Q

Anti-HSD3B1 Antibody - LS-C153359 from LifeSpan Bioscience

Prices $305.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HSD3B1 Antibody (aa26-55, APC) - LS-C236407 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HSD3B1 Antibody (aa26-55, FITC) - LS-C236410 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HSD3B1 Antibody (aa26-55, AP) - LS-C236408 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HSD3B1 Antibody (aa26-55, Biotin) - LS-C236409 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HSD3B1 Antibody (aa26-55, PE) - LS-C236415 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HSD3B1 Antibody - LS-C311239 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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