Anti-HSD3B1 antibody [EPR9686]

• Supplier: Abcam
• Catalog: ab150384
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9686
• Applications: WB IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: HSD3B1
• Conjugate: Unconjugated

Product images

Overlay histogram showing JEG3 cells stained with ab150384 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150384, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HSD3B1 antibody [EPR9686] (ab150384) at 1/1000 dilution + Human placenta cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit antibody at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human bladder carcinoma labeling HSD3B1 with ab150384 at a 1/50 dilution.