Anti-HSD3B2 antibody [373CT9.1.3]
| Name | Anti-HSD3B2 antibody [373CT9.1.3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab75710 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 373CT9.1.3 |
| Applications | WB FC |
| Species Reactivities | Human, Mouse, Rat, Sheep, Goat, Horse, Bovine, Cat, Dog, Pig, Monkey, Monkey, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HSD3B2 |
| Description | Mouse Monoclonal |
| Gene | HSD3B1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/4527_ab75710-4-ab75710FC.jpg)
Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Adrenal (Human) Whole Cell Lysate - adult normal tissue (ab29249) at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/4528_HSD3B2-Primary-antibodies-ab75710-3.jpg)
Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Adrenal (Human) Whole Cell Lysate - adult normal tissue (ab29249) at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Lymphatic involvement in the disappearance of steroidogenic cells from the corpus - Lymphatic involvement in the disappearance of steroidogenic cells from the corpus
Abe H, Al-zi'abi MO, Sekizawa F, Acosta TJ, Skarzynski DJ, Okuda K. PLoS One. 2014 Feb 20;9(2):e88953.
Expression of aldo-keto reductase 1C23 in the equine corpus luteum in different - Expression of aldo-keto reductase 1C23 in the equine corpus luteum in different
Kozai K, Hojo T, Tokuyama S, Szostek AZ, Takahashi M, Sakatani M, Nambo Y, Skarzynski DJ, Okuda K. J Reprod Dev. 2014 Apr 24;60(2):150-4. Epub 2014 Feb 4.