Anti-hSET1 antibody - ChIP Grade
| Name | Anti-hSET1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab70378 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ChIP ChIP ChIP WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Horse, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Hamster, Orangutan, Elephant |
| Antigen | Synthetic peptide mapping to a region between residues 1200 and 1250 of human hSET1 (TrEMBL entry O15047) |
| Description | Rabbit Polyclonal |
| Gene | SETD1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling hSET1 with ab70378 at 1/1000 (1µg/ml). Detection: DAB.
Lanes 1 - 4 : Anti-hSET1 antibody - ChIP Grade (ab70378) at 1 µg/mlLanes 5 - 8 : Anti-hSET1 antibody - ChIP Grade (ab70378) at 0.2 µg/mlLane 1 : 293T whole cell lysate at 100 µgLane 2 : 293T whole cell lysate at 200 µgLane 3 : 293T nuclear extract at 100 µgLane 4 : 293T nuclear extract at 200 µgLane 5 : 293T whole cell lysate at 100 µgLane 6 : 293T whole cell lysate at 200 µgLane 7 : 293T nuclear extract at 100 µgLane 8 : 293T nuclear extract at 200 µgdeveloped using the ECL technique
Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
ab70378 (1/400) staining hSET1 in assynchronous HeLa cells (green). Cells were paraformaldehyde-fixed, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.See Abreview
Product References
GRHL3/GET1 and trithorax group members collaborate to activate the epidermal - GRHL3/GET1 and trithorax group members collaborate to activate the epidermal
Hopkin AS, Gordon W, Klein RH, Espitia F, Daily K, Zeller M, Baldi P, Andersen B. PLoS Genet. 2012;8(7):e1002829.
LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. - LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing.
Le Douce V, Colin L, Redel L, Cherrier T, Herbein G, Aunis D, Rohr O, Van Lint C, Schwartz C. Nucleic Acids Res. 2012 Mar;40(5):1904-15.
.