![Overlay histogram showing HepG2 cells stained with ab119398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119398, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5148_ab119398-8-ab119398FC.jpg)
Overlay histogram showing HepG2 cells stained with ab119398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119398, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Hsp75 antibody [1A1] (ab119398) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hsp75Lysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5149_Hsp75-Primary-antibodies-ab119398-1.jpg)
All lanes : Anti-Hsp75 antibody [1A1] (ab119398) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hsp75Lysates/proteins at 5 µg per lane.
![All lanes : Anti-Hsp75 antibody [1A1] (ab119398) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5150_Hsp75-Primary-antibodies-ab119398-2.jpg)
All lanes : Anti-Hsp75 antibody [1A1] (ab119398) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using ab119398 at a dilution of 1/150.
mmunohistochemical staining of paraffin-embedded Human endometrium tissue using ab119398 at a dilution of 1/150.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using ab119398 at a dilution of 1/150.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using ab119398 at a dilution of 1/150.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp75 using ab119398 at a dilution of 1/100.