All lanes : Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112) at 3 µg/mlLane 1 : Human heart lysateLane 2 : Mouse heart lysateLane 3 : Rat heart lysate
Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112) at 2 µg/ml + Rat heart lysateObserved band size : 43 kDa (why is the actual band size different from the predicted?)Additional bands at : 65 kDa,80 kDa. We are unsure as to the identity of these extra bands.
ab22112 at a 1/200 dilution staining HtrA3 in human bronchial cell line BEAS-2B by Immunocytochemistry/ Immunofluorescence. Cells were treated with 20µM etoposide for 24 hours.