Anti-htrA1 antibody
| Name | Anti-htrA1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38611 |
| Prices | $400.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human htrA1 aa 116-147 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Human htrA1 peptide |
| Description | Rabbit Polyclonal |
| Gene | HTRA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-htrA1 antibody (ab38611) + Mouse brain tissue lysate.
ab38611 staining htrA1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling htrA1 with ab38611. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling htrA1 (green) with ab38611. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).
ICC/IF image of ab38611 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Impact of age on the cerebrovascular proteomes of wild-type and Tg-SwDI mice. - Impact of age on the cerebrovascular proteomes of wild-type and Tg-SwDI mice.
Searcy JL, Le Bihan T, Salvadores N, McCulloch J, Horsburgh K. PLoS One. 2014 Feb 26;9(2):e89970.
Inflammatory markers associated with osteoarthritis after destabilization surgery - Inflammatory markers associated with osteoarthritis after destabilization surgery
Larkin DJ, Kartchner JZ, Doxey AS, Hollis WR, Rees JL, Wilhelm SK, Draper CS, Peterson DM, Jackson GG, Ingersoll C, Haynie SS, Chavez E, Reynolds PR, Kooyman DL. Front Physiol. 2013 May 28;4:121.
Identification and regulation of bone morphogenetic protein antagonists - Identification and regulation of bone morphogenetic protein antagonists
Fenwick MA, Mansour YT, Franks S, Hardy K. Endocrinology. 2011 Sep;152(9):3515-26.
Regulation of sororin by Cdk1-mediated phosphorylation. - Regulation of sororin by Cdk1-mediated phosphorylation.
Dreier MR, Bekier ME 2nd, Taylor WR. J Cell Sci. 2011 Sep 1;124(Pt 17):2976-87.
Role of HTRA1, a serine protease, in the progression of articular cartilage - Role of HTRA1, a serine protease, in the progression of articular cartilage
Polur I, Lee PL, Servais JM, Xu L, Li Y. Histol Histopathol. 2010 May;25(5):599-608.