Overlay histogram showing HeLa cells stained with ab75982 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75982, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor® 488 (IgG H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5614_htra1.gif)
All lanes : Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded human colon using ab75982 at 1/100-1/250 dilution.
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