Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysateObserved band size : 190 kDa (why is the actual band size different from the predicted?)Additional bands at : 120 kDa,130 kDa. We are unsure as to the identity of these extra bands.
ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).